flowAI report

Quality control analysis

Summary

The anomalies were removed from: Flow Rate, Flow Signal and Flow Margin
Anomalies detected in total: 33.29 %
Number of high quality events: 598101

Flow rate check

32.11 % anomalies detected in the flow rate check.

The plot reconstructs the flow rate with a resolution of 1/10 of a second. Anomalies in the flow rate are identified with an algorithm based on the generalied ESD outlier detection method. The anomalies are circled in green.

Signals acquisition check

28.67 % anomalies detected in the signal aquisition check.

The more stable region selected consistent for all channels is highlighted in yellow. If the removal of outliers has been required before the execution of the changepoint analysis, the detected outliers are cicled in green.

The FCS file was divided in 1794 bins, hence each bin contains 500 event(s). The stable region is located between the bins 1 and 1279.

More info on the changepoints detected

Changepoints detected in channels with shifts in signal acquisition:

##                 1
## 488 525/50-A 1281
## 488 715/30-A 1279
## 405 450/50-A 1285
## 405 515/20-A 1285
## 405 605/40-A 1281
## 405 670/30-A 1281
## 405 710/40-A 1281
## 405 780/60-A 1281
## 355 379/28-A 1281
## 355 515/30-A 1281
## 355 560/40-A 1281
## 355 740/35-A 1281
## 355 820/60-A 1281
## 640 670/30-A 1281
## 640 730/35-A 1285
## 640 780/60-A 1281
## 561 585/15-A 1281
## 561 610/20-A 1281
## 561 780/60-A 1281
## 405 585/15-A 1281

Channels excluded from the analysis: FSC-A, FSC-W, SSC-A, SSC-W.

Dynamic range check

1.22 % anomalies detected in dynamic range check.

The plot shows where the anomalies occured the most. The x-axis scale is complementary to the one of the signal acquisition plot.

More info on the anomalies detected in the dynamic range check

For each channel analyzed, the table shows the number of events that did not pass the dynamic range check:

##              lower_range upper_range
## 488 525/50-A          17         795
## 488 715/30-A          16          41
## 405 450/50-A          10         167
## 405 515/20-A           0        2040
## 405 605/40-A           2         164
## 405 670/30-A          27         143
## 405 710/40-A          36          91
## 405 780/60-A          74          26
## 355 379/28-A          21         130
## 355 515/30-A          15          97
## 355 560/40-A          18         212
## 355 740/35-A           1          33
## 355 820/60-A          56           6
## 640 670/30-A           4          10
## 640 730/35-A           5           9
## 640 780/60-A           8          17
## 561 585/15-A           8        9468
## 561 610/20-A          18        5371
## 561 780/60-A           9          52
## 405 585/15-A           4         174
## total_SUM            349       19046
## total_UNIQUE         274       10681